Recombinant Human Cyclin-dependent Kinase Inhibitor 1A (p21, Cip1)

• Cat.No.: CDKN1A-169H
• Product Overview: Recombinant Human p21WAF1 protein expressed inE.coliencodes full length amino acids and has a molecular mass of 47 kDa.

Specification

• Cat. No.: CDKN1A-169H
• Description: p21WAF1/Cip1/Sdi1/Pic1 is a tumor suppressor protein. Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21WAF1. p21WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.
• Source: Recombinant full length human p21WAF1 protein expressed inE.coli.
• Formulation: 2µg/20µl,100µg/1ml purified human p21WAF1 protein in 50mM Tris-Acetate pH-7.5, 1mM EDTA and 20% Glyecrol.
• Application And Suggested Dilutions: •ELISA •Inhibition Assays •Western Blotting
• Characterization: On SDS-PAGE commassie blue staining gel, the purified recombinant protein shows a band of 47kDa.
• Activity: Not known.
• Storage & Stability: Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months.

Gene Information

• Gene Name: CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) [ Homo sapiens ]
• Synonyms: CDKN1A; cyclin-dependent kinase inhibitor 1A (p21, Cip1) ; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; MDA6; PIC1; p21Cip1/Waf1; p21CIP1; cyclin-dependent kinase inhibitor 1A; OTTHUMP00000016298; DNA synthesis inhibitor; CDK-interaction protein 1; wild-type p53-activated fragment 1; melanoma differentiation associated protein 6
• Gene ID: 1026
• mRNA Refseq: NM_000389
• Protein Refseq: NP_000380
• MIM: 116899
• UniProt ID: P38936
• Chromosome Location: 6p21.1
• Pathway: Bladder cancer; Cell cycle; Chronic myeloid leukemia; ErbB signaling pathway; Glioma; Melanoma; Pathways in cancer; Prostate cancer; p53 signaling pathway; Cell Cycle Checkpoints; Cell Cycle, Mitotic; DNA Replication
• Function: cyclin binding; cyclin-dependent protein kinase inhibitor activity; cyclin-dependent protein kinase inhibitor activity; metal ion binding; protein complex binding; protein kinase inhibitor activity; zinc ion binding