Recombinant Human Pancreatic And Duodenal Homeobox 1

• Cat.No.: PDX1-176H
• Product Overview: Recombinant Human Insulin promoter factor-1 Protein, produced inE. coli, contains 283 amino acids and has a molecular mass of 30.64 kDa.

Specification

• Cat. No.: PDX1-176H
• Description: Pancreatic Duodenal Homeobox-1 activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. binds preferentially the dna motif 5"-[ct]taat[tg]-3". During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. at adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. Mutations in this gene may be involved in several disorders of the pancreas or in diabetes mellitus.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Formulation: Lyophilized Powder of liquid in PBS, pH 7.4.
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Solubility: It is recommended to reconstitute the lyophilized PDX1 in sterile PBS, 10% glycerol to prepare a stock of 100μg/ml.
• Biological Acivity: The activity of human PDX-1 is determined by the ability to stimulate NeuroD1 gene expression in WB cells and binding to insulin promoter be EMSA assay.
• Stability: Recombinant Human PDX1 although stable at 25°C 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PDX1 pancreatic and duodenal homeobox 1 [ Homo sapiens ]
• Synonyms: GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1; PDX1; IUF-1; IPF-1;Pancreas/duodenum homeobox protein 1; Insulin promoter factor 1; Islet/duodenum homeobox-1; Somatostatin-transactivating factor 1; Insulin upstream factor 1; Glucose-sensitive factor; pancreatic and duodenal homeobox 1; glucose-sensitive factor; insulin upstream factor 1; somatostatin transcription factor 1; pancreatic-duodenal homeobox factor 1; homeodomain transcription factor
• Gene ID: 3651
• mRNA Refseq: NM_000209
• Protein Refseq: NP_000200
• MIM: 600733
• UniProt ID: P52945
• Chromosome Location: 13q12.1
• Pathway: Maturity onset diabetes of the young; Type II diabetes mellitus; Regulation of beta-cell development
• Function: protein binding; sequence-specific DNA binding; specific RNA polymerase II transcription factor activity; transcription activator activity; transcription factor activity