Active Recombinant Human TNFSF13B

• Cat.No.: TNFSF13B-135H
• Product Overview: Recombinant Human tumor necrosis factor (ligand) encoding the human BAFF protein sequence (containing the signal peptide sequence and the mature BAFF sequence) was expressed in modifiedhuman 293 cells.

Specification

• Cat. No.: TNFSF13B-63H
• Description: BAFF is a type II membrane glycoprotein expressed by T cells, dendritic cells, macrophages and neutrophils. BAFF is predominantly a B cell survival factor and specifically promotes the proliferation and survival of activated B cells by inducing expression of pro-survival oncogenes including Bcl-xL, Bcl-2 and Mcl-1. BAFF also mediates Ig switching to IgD as well as B cell maturation, suggesting it plays a role in humoral immune responses.
• Source: Human 293 cells.
• Amino Acid Sequence: AVQGPEETVTQDCLQLIADSETPTI QKGSYTFVPWLLSFKRGSALEEKEN KILVKETGYFFIYGQVLYTDKTYAM GHLIQRKKVHVFGDELSLVTLFRCI QNMPETLPNNSCYSAGIAKLEEGDE LQLAIPRENAQISLDGDVTFFGALK LL.
• Molecular Mass: BAFFB migrates as a band between 15 and 20 kDa in SDS-PAGE. This compares with the predicted molecular mass of 17 kDa.
• pI: Due to post-translational modifications the Symansis BAFFB separates into a number of glycoforms with a pI between 4.7 and 5.5 on 2D PAGE. This is in agreement with the unmodified BAFF that has a predicted pI of 4.9.
• Purity: >95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Activity: The ED₅₀of BAFFB is typically 5-10 ng/ml as measured by its ability to neutralise dexamethasone toxicity using the RPMI 8226 cell line.

Gene Information

• Gene Name: TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b [ Homo sapiens ]
• Synonyms: TNFSF13B; tumor necrosis factor (ligand) superfamily, member 13b; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20; TNFSF13B;Tumor necrosis factor ligand superfamily member 13B;TNF- and APOL-related leukocyte expressed ligand 1; B lymphocyte stimulator; B cell-activating factor; Dendritic cell-derived TNF-like moleculeCD_antigen=CD257;Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosisfactor ligand superfamily member 13b, soluble form
• Gene ID: 10673
• mRNA Refseq: NM_001145645
• Protein Refseq: NP_001139117
• UniProt ID: Q9Y275
• Chromosome Location: 13q32-q34
• MIM: 603969
• Pathway: Cytokine-cytokine receptor interaction
• Function: cytokine activity; tumor necrosis factor receptor binding