| Cat. No. : |
TDGF1-496H |
| Description : |
Cripto-1 (CR-1), also known as teratocarcinoma-derived growth factor-1 (TDGF-1), is a small glycoprotein that contains a single divergent EGF-like motif as well as a novel cysteine-rich domain termed the Cripto motif. It is the founding member of the EGF-CFC gene family, which is conserved among vertebrates, with homologs in chick, frogs, and zebrafish. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
LGHQEFARPSRGYLAFRDDSIWPQE EPAIRPRSSQRVPPMGIQHSKELNR TCCLNGGTCM LGSFCACPPSFYGRNCEHDVRKENC GSVPHDTWLPKKCSLCKCWHGQLRC FPQAFLPG CDGLVMDEHLVASRTPELPPSGSSN TKVDKKVEPKSCDKTHTCPPCPAPE LLGGPSVFLFP PKPKDTLMISRTPEVTCVVVDVSHE DPEVKFNWYVDGVEVHNAKTKPREE QYNSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAP IEKTISKAKGQPREPQVYTLPPSRD ELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKT TPPVLDSDGSFFLYSKLTVDKSRWQ QGNVFSCSVMH EALHNHYTQKSLSLSPGK. |
| Molecular Mass : |
Under reducing conditions Cripto-1 – Fc Chimera migrates as a broad band between 45 and 55 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified Cripto-1 - Fc Chimera that has a predicted monomeric molecular mass of 42.8 kDa. |
| pI : |
Cripto-1 – Fc Chimera separates into a number of glycoforms with an observed pI between 6.5 and 10 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Cripto-1 - Fc Chimera that has a predicted pI of 8.2. |
| % Carbohydrate : |
Purified Cripto-1 – Fc Chimera consists of 5-30% carbohydrate by weight. |
| Glycosylation : |
Cripto-1 – Fc Chimera contains N-linked and O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by Coomassie Briliant Blue. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
| Activity : |
200 ng/ml Cripto-1 – Fc Chimera induces ERK1 and ERK2 phosphorylation in human umbilical vein endothelial (HUVEC) cells |
