| Cat. No. : |
FLT3-140H |
| Description : |
Fms-like tyrosine kinase 3 (Flt-3) and its cognate ligand, Flt-3 Ligand (Flt-3L), are essential growth factors involved in the survival and differentiation of hematopoietic progenitor and stem cells, specifically the development of NK cells, pre-B and pre-T cells, monocytes/macrophages and dendritic cells. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
NQDLPVIKCVLINHKNNDSSVGKSS SYPMVSESPEDLGCALRPQSSGTVY EAAAVEVDVSASITLQVLVDAPGNI SCLWVFKHSSLNCQPHFDLQNRGVV SMVILKMTETQAGEYLLFIQSEATN YTILFTVSIRNTLLYTLRRPYFRKM ENQDALVCISESVPEPIVEWVLCDS QGESCKEESPAVVKKEEKVLHELFG TDIRCCARNELGRECTRLFTIDLNQ TPQTTLPQLFLKVGEPLWIRCKAVH VNHGFGLTWELENKALEEGNYFEMS TYSTNRTMIRILFAFVSSVARNDTG YYTCSSSKHPSQSALVTIVEKGFIN ATNSSEDYEIDQYEEFCFSVRFKAY PQIRCTWTFSRKSFPCEQKGLDNGY SISKFCNHKHQPGEYIFHAENDDAQ FTKMFTLNIRRKPQVLAEASASQAS CFSDGYPLPSWTWKKCSDKSPNCTE EITEGVWNRKANRKVFGQWVSSSTL NMSEAIKGFLVKCCAYNSLGTSCET ILLNSPGPFPFIQDRIPKVDKKVEP KSCDKTHTCPPCPAPELLGGPSVFL FPPKPKDTLMISRTPEVTCVVVDVS HEDPEVKFNWYVDGVEVHNAKTKPR EEQYNSTYRVVSVLTVLHQDWLNGK EYKCRVSNKALPAPIEKTISKAKGQ PREPQVYTLPPSRDELTKNQVSLTC LVKGFYPSDIAVEWESNGQPENNYK TTPPVLDSDGSFFLYSKLTVDKSRW QQGNVFSCSVMHEALHNHYTQKSLS LSPGK. |
| Molecular Mass : |
Flt-3-Fc Chimera migrates as a broad band between 100 and 130 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. |
| pI : |
Flt-3-Fc Chimera separates into a number of isoforms with a pI between 5.1 and 6.8 in 2D PAGE due to post-translational modifications, in particular glycosylation. |
| % Carbohydrate : |
Purified Flt-3-Fc Chimera consists of 15-35% carbohydrate by weight. |
| Glycosylation : |
Flt-3-Fc Chimera has N-linked and O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
| Activity : |
The ED50 of Flt-3-Fc Chimera is typically 1.0 -2.0 ng/ml as measured by its ability to neutralize Flt-3 mediated proliferation of the OCI/AML5 human leukemia cell line. |
