| Cat. No. : |
IL2-116H |
| Description : |
Interleukin-2 (IL-2) is a pleotropic glycoprotein that mediates the production of regulatory T cells and promotes T cell dependent tolerance. Additionally, IL-2 promotes the proliferation of antigen-activated T lymphocytes, facilitates the cytolytic activity of natural killer (NK) cells and stimulates anti-tumor activity in monocytes by inducing synthesis of GM-CSF, IL-1b and IL-6. IL-2 also promotes the proliferation of large granular lymphocytes and the growth and differentiation of mitogen activated B lymphocytes in vitro. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
APTSSSTK KTQLQL EHLLLDLQ MILNGIN NYKNPKLT RMLTFKFY MPKKATEL KHLQCLEE ELKPLEEVL NLAQSK NFHLRPRD LISNINVIVLELKG SETTF MCEY ADE TATIVEFL NRWITFCQSIISTLT. |
| Molecular Mass : |
IL-2 migrates as a band between 15 and 18 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL-2 that has a predicted molecular mass of 15.4 kDa. |
| PI : |
IL-2 separates into a number of isoforms with a pI between 6.0 and 7.4 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-2 that has a predicted pI of 7.05. |
| % Carbohydrate : |
Purified IL-2 consists of 0-15% carbohydrate by weight. |
| Glycosylation : |
IL-2 has O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
| Activity : |
The ED50of IL-2 is typically 0.15- 0.25 ng/ml as measured in a cell proliferation assay using the IL-2 dependent murine CTLL-2 T cell line. |
