| Cat. No. : |
IL5RA-127H |
| Description : |
Interleukin 5 (IL-5) plays an important role in B cell development by inducing the terminal differentiation of late-developing B cells. Additionally, IL-5 regulates the differentiation, survival and degranulation of eosinophils. IL-5 is expressed predominately by T-lymphocytes and mast cells, and to a lesser extent by eosinophils, natural killer cells and endothelial cells. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
DLLPDEKISLLPPVNF TIKVTGLAQVLL QWKP NPDQ EQRN VNLEYQVKIN APKEDDYET RITES KCVTIL HKGFSASVRTILQNDHSLLASSWASA EL HAPPGSPG TSIVNLTCTTN T TEDNYSRLRSY QVSLHCTWLVG TDAPEDTQYFL YYR YGS WTEEC QEYSKDTLGRN IACWFPRTFILS KG RD WLAVLV NGSSKHSAIRP FDQL FAL HAID QINPP LNVTA EIEGTRLSIQW EKPVSAFPIHC FDY EVKIHNTR NGYL QIEKLMT NAFISIIDDLSKY DVQVRAAV SSMCREAG LWSEWSQ RIPKVDKKV EP KSCDKT HTCPPCPA PELLGGPS VFLFPPKP KDTLMISRTPE VTCVVVD VS HEDPE VK FNWYV DGVEVHN AKTKPREEQYNST YRVVSVLT VLHQDWL NG KE YKCRVSNKALPAPI EK TIS KA K GQPREPQ VYTLPPSR DELT KNQ VSLTC LVK GFYPSD IAVEWESNG QPENNYK TTP PVL DS DGS FFLY SKLT VDK SRW QQG NVFSCSVMHEALHNHYTQKSLSLSPGK. |
| Molecular Mass : |
Under reducing conditions IL-5R alpha (322aa) – Fc Chimera migrates as a broad band between 65 and 85 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified IL-5R alpha (322aa) - Fc Chimera that has a predicted monomeric molecular mass of 64 kDa. |
| PI : |
IL-5R alpha (322aa) – Fc Chimera separates into a number of isoforms with a pI between 5.5 and 7.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-5R alpha (322aa) – Fc Chimera that has a predicted pI of 6.2. |
| % Carbohydrate : |
Purified IL-5R alpha (322aa) – Fc Chimera consists of 5-30% carbohydrate by weight. |
| Glycosylation : |
IL-5R alpha (322aa) – Fc Chimera contains N- and O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
| Activity : |
The ED50of IL-5R alpha (322aa) – Fc Chimera is typically 0.25 - 0.4 ug/ml as measured by its ability to neutralize IL-5 mediated proliferation of the human growth-factor dependent TF-1 cell line. |
