| Cat. No. : |
TNFRSF4-455H |
| Description : |
Ox40 is a member of the tumor necrosis factor receptor (TNFR) family. It is a type I transmembrane protein expressed primarily on the surface of activated CD4+ T-cells. It is also expressed at low levels on the surface of activated CD8+ T cells, B cells, dendritic cells and eosinophils. Ox40 is a co-stimulatory molecule involved in T-cell activation and proliferation, the induction of cytokine production by effector T-cells, generation of memory T-cells, and arresting peripheral T-cell tolerance in vivo. Expression of Ox40 is induced following the initiation of a CD28 signal. It has been reported that the interaction of Ox40 with its ligand plays a role in the expansion of T-cell numbers at the height of the immune response as well as the generation of memory T-cells. Human Ox40 comprises a 186 amino acid extracellular domain containing four TNFR-Cys repeats, followed by a 21 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain. Ox40 activity is mediated by NF-kappaB signaling via interaction of the intracellular domain of Ox40 with TRAF proteins. |
| Source : |
human 293 cells. |
| Molecular Mass : |
Ox40-Fc Chimera igrates as a broad band between 55 and 65 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified Ox40-Fc Chimera that has a predicted molecular mass of 46.4kDa. |
| PI : |
Ox40-Fc Chimera separates into a number of isoforms with a pI between 5.5 and 7.9 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Ox40-Fc Chimera that has a predicted pI of 8.32. |
| % Carbohydrate : |
purified Ox40-Fc Chimera consists of 15-30% carbohydrate by weight. |
| Glycosylation : |
Ox40-Fc Chimera has N-linked and O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. |
