Recombinant Human CCL5 was expressed in HEK293.
Chemokine (C-C motif) ligand 5(CCL5), also known as RANTES (Regulated upon activation, Normal T cell Expressed and presumable Secreted) is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. RANTES is chemotactic for T cells, eosinophils, and basophils, and plays an active role in recruiting leukocytes in inflammatory sites. With the help of specific cytokines (i.e., IL-2 and IFN-γ) that are released by T cells, RANTES induces the proliferation and activation of certain natural-killer (NK) cells to form CHAK (CC-Chemokine-activated killer) cells. RANTES is also an HIV-suppressive factor released from CD8+ T cells. This chemokine has been localized to chromosome 17 in humans. It has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV).
Lyophilized after extensive dialysis against PBS.
The EC50 value of human RANTES/CCL5 on Ca2+ mobilization assay in CHO-K1/Gα15/hCCR1 cells (human Gα15 and human CCR1 stably expressed in CHO-K1 cells) is less than 0.2 μg/ml.
8 kDa, observed by reducing SDS-PAGE.
68 amino acids
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS
< 0.2 EU/μg, determined by LAL method.
> 98% as analyzed by SDS-PAGE.
Lyophilized recombinant human RANTES/CCL5 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, human RANTES/CCL5 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade
Reconstituted in ddH2O or PBS at 100 μg/ml.