| Cat. No. : |
TNFRSF1A-152H |
| Description : |
Tumor necrosis factor alpha (TNF-a ; TNF alpha) and the structurally related lymphotoxin alpha are pro-inflammatory cytokines produced by a variety of immune and non-immune cells in response to multiple stimuli. These stimuli can include bacteria and bacterial products such as LPS, viral infections, T-cell receptor activation and cross-linking of surface immunoglobulin (Ig) on B cells. Expression of TNF-alpha has been detected in macrophages/monocytes, B-cells, T-cells, basophils, eosinophils, glial cells and mast cells. The effects of TNF-a are mediated through the TNF receptors, TNFRI (TNF R1) and TNFRII (TNF R2). TNFRI is a 55 kDa glycoprotein and contains 5 N-linked glycosylation sites. |
| Source : |
human 293 cells. |
| Molecular Mass : |
TNF RI-Fc Chimera migrates as a band between 50 and 60 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified TNF RI-Fc Chimera that has a predicted molecular mass of 48.0kDa. |
| PI : |
TNF RI-Fc Chimera separates into a number of isoforms with a pI between 6.5 and 8.6 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified TNF RI-Fc Chimera that has a predicted pI of 7.69. |
| % Carbohydrate : |
TNF RI-Fc Chimera consists of 5-20% carbohydrate by weight. |
| Glycosylation : |
TNF RI-Fc Chimera has N-linked and possibly O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. |
| Activity : |
The ND50of TNF RI-Fc Chimera is typically 15-30 ng/ml as measured by its ability to neutralize TNF-a mediated cytoxicity in murine WEHI 164 cells in the presence of actinomycin D. |
