| Cat. No. : |
VEGFC-157H |
| Description : |
Vascular endothelial growth factor C (VEGF-C), also known as Flt4 ligand (Flt4-L), vascular endothelial growth factor-related protein (VRP) and VEGF-2, is a member of the cysteine-knot growth factor superfamily and exhibits homology with VEGF-121 (32%) and PDGF (27%). However VEGF-C also possesses a 180 amino acid C-terminal cysteine-rich domain absent in other VEGFs. The major secreted form of VEGF-C is a disulfide linked antiparallel homodimeric protein containing 5 potential N-linked glycosylation sites and has a theoretical molecular weight of 44 kDa. VEGF-C is predominantly involved in angiogenesis, vasculogenesis, lymphangiogenesis as well as an inhibitor of dendritic cell maturation. VEGF-C also possesses both mitogenic and chemotactic activity w.r.t. endothelial cells and monocytes. |
| Source : |
human 293 cells. |
| Molecular Mass : |
VEGF-C secreted antiparallel homodimer migrates between 45 and 55 kDa due to post-translational modifications, in particular glycosylation. This compares with the unmodified homodimer that has a predicted molecular mass of 43.9 kDa. Individual chains of VEGF-C migrate as a band between 27 and 35 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified VEGF-C chains that have a predicted molecular mass of 21.6 and 22.2 kDa. |
| PI : |
VEGF-C secreted antiparallel homodimer separates into a number of isoforms with a pI between 5.6 and 8.4 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified VEGF-C homodimer that has a predicted pI of 7.8. |
| % Carbohydrate : |
VEGF-C consists of 15-40% from 1D gel of individual chains carbohydrate by weight. |
| Glycosylation : |
VEGF-C contains N- and probably O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. |
