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Recombinant Human D-amino-acid Oxidase, His-tagged

Cat.No. : DAO-476H
Product Overview : Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 41.6 kDa (367aa).
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Cat. No. : DAO-476H
Description : D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging.
Source : E.coli.
Amino Acid Sequence : MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL.
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT.
Purity : > 95% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : DAO D-amino-acid oxidase [ Homo sapiens ]
Synonyms : DAO; D-amino-acid oxidase; DAAO; OXDA; DAMOX; MGC35381; EC 1.4.3.3
Gene ID : 1610
mRNA Refseq : NM_001917
Protein Refseq : NP_001908
MIM : 124050
UniProt ID : P14920
Chromosome Location : 12
Pathway : Arginine and proline metabolism; D-Arginine and D-ornithine metabolism; Glycine, serine and threonine metabolism; Metabolic pathways
Function : D-amino-acid oxidase activity; binding; oxidoreductase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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