Recombinant Human SAR1 Homolog A (S. cerevisiae) , His-tagged

• Cat.No.: SAR1A-479H
• Product Overview: Recombinant human SAR1A, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventionalchromatography techniques. MW = 24.5 kDa (218aa).

Specification

• Cat. No.: SAR1A-479H
• Description: SAR1a gene homolog 1, also known as SAR1A, belongs to the small GTPase superfamily. Sar1 is an essential component of COPII vesicle coats involved in export of cargo from the ER. The GTPase activity of Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. GDP-bound Sar1 interacts with the membrane-bound exchange factorSec12and exchanges its bound GDP for GTP.
• Source: E.coli.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSFIFEWIYN GFSSVLQFLG LYKKSGKLVF LGLDNAGKTT LLHMLKDDRL GQHVPTLHPT SEELTIAGMT FTTFDLGGHE QARRVWKNYL PAINGIVFLV DCADHSRLVE SKVELNALMT DETISNVPIL ILGNKIDRTD AISEEKLREI FGLYGQTTGK GNVTLKELNA RPMEVFMCSV LKRQGYGEGF RWLSQYID
• Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SAR1A SAR1 homolog A (S. cerevisiae) [ Homo sapiens ]
• Synonyms: SAR1A; SAR1 homolog A (S. cerevisiae); SAR1; Sara; SARA1; masra2; OTTHUMP00000019744; OTTHUMP00000019745; OTTHUMP00000019748; SAR1 gene homolog A; SAR1a gene homolog (S. cerevisiae) 1;SARA; GTP-binding protein SAR1a; COPII-associated small GTPase; OTTHUMP00000019743; OTTHUMP00000060603; SAR1a gene homolog 1
• Gene ID: 56681
• mRNA Refseq: NM_001142648
• Protein Refseq: NP_001136120
• MIM: 607691
• UniProt ID: Q9NR31
• Chromosome Location: 10q22.1
• Function: GTP binding; GTPase activity; nucleotide binding