Recombinant Human Phosphatase And Tensin Homolog

• Cat.No.: PTEN-482H
• Product Overview: Recombinant PTEN protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 49.3 kDa (423aa)

Specification

• Cat. No.: PTEN-482H
• Description: PTEN, also known as phosphatase and tensin homolog, is a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein acts as both a dual-specificity protein phosphatase and a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate. PTEN negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
• Source: E.coli.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTAIIKEIVS RNKRRYQEDG FDLDLTYIYP NIIAMGFPAE RLEGVYRNNI DDVVRFLDSK HKNHYKIYNL CAERHYDTAK FNCRVAQYPF EDHNPPQLEL IKPFCEDLDQ WLSEDDNHVA AIHCKAGKGR TGVMICAYLL HRGKFLKAQE ALDFYGEVRT RDKKGVTIPS QRRYVYYYSY LLKNHLDYRP VALLFHKMMF ETIPMFSGGT CNPQFVVCQL KVKIYSSNSG PTRREDKFMY FEFPQPLPVC GDIKVEFFHK QNKMLKKDKM FHFWVNTFFI PGPEETSEKV ENGSLCDQEI DSICSIERAD NDKEYLVLTL TKNDLDKANK DKANRYFSPN FKVKLYFTKT VEEPSNPEAS SSTSVTPDVS DNEPDHYRYS DTTDSDPENE PFDEDQHTQI TKV.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 2mM DTT, 100Mm NaCl, and 20% glycerol.
• Purity: > 85% by SDS – PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PTEN phosphatase and tensin homolog [ Homo sapiens ]
• Synonyms: PTEN; phosphatase and tensin homolog; PTEN phosphatase and tensin homolog; phosphatase and tensin homolog; mutated in multiple advanced cancers 1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; EC 3.1.3.16; EC 3.1.3.48; EC 3.1.3.67; MMAC1; TEP1; PTEN1; Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; 10q23del; BZS; MGC11227; MHAM; OTTHUMP00000020032; Mutated in multiple advanced cancers 1; phosphatase and tensin homolog
• Gene ID: 5728
• mRNA Refseq: NM_000314
• Protein Refseq: NP_000305
• MIM: 601728
• UniProt ID: P60484
• Chromosome Location: 10q23
• Pathway: Endometrial cancer; Focal adhesion; Glioma; Inositol phosphate metabolism; Melanoma; Pathways in cancer; Phosphatidylinositol signaling; system; Prostate cancer; Small cell lung cancer; Tight junction; p53 signaling pathway; Signaling in Immune system; Signalling by NGF
• Function: PDZ domain binding; hydrolase activity; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; lipid binding; magnesium ion binding; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3-phosphatase activity; platelet-derived growth factor receptor binding; protein binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity