| Cat. No. : |
FGF18-171H |
| Description : |
Fibroblast Growth Factor-18 (FGF-18) is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. |
| Source : |
E.Coli. |
| Supplied : |
Sterile filtered and the the protein was lyophilized from 10mM Tris, pH8.0 (no additives). |
| Purity : |
>95% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of FGF-18. |
| Reconstitution : |
The lyophilized FGF-18 should be reconstituted in 5mM TRIS, pH8.0 to a concentration of 0.1-1.0mg.ml. Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions and stored at 4°Cfor up to 1 week or stored at – 20℃ for future use. For most in-vitro applications, human FGF-18 exerts its biological activity in the concentration range of 0.1 to 20ng/ml. |
| Note : |
If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration greater than 10ug/ml. |
| Reactivity : |
The biological activity was determined by the does- dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 <0.5ng/ml, corresponding to a specific activity of >1 X 106 (1 million) units/mg. |
| Amino Acid Sequence : |
MAEENVDFRI HVENQTRARD DVSRKQLRLY QLYSRTSGKH IQVLGRRISA RGEDGDKYAQ LLVETDTFGS QVRIKGKETE FYLCMNRKGK LVGKPDGTSK ECVFIEKVLE NNYTALMSAK YSGWYVGFTK KGRPRKGPKT RENQQDVHFM KRYPKGQPE LQKPFKYTTV TKRSRRIRP THPAAHFIKR LYQGQLPFP NHAEKQKQFE FVGSAPTRRTK RTRR PQPLT |
| Storage : |
The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted human FGF-18 should be stored in working aliquots at -20°C. |
