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Recombinant Human Fibroblast Growth Factor 5

Cat.No. : FGF5-172H
Product Overview : This recombinant human FGF-5 is a 27.6 kDa protein containing 252 amino acid residues and it was expressed inE.Coli.
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Cat. No. : FGF5-172H
Description : Fibroblast Growth Factor-5 (FGF-5) , is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors.
Source : E.Coli.
Supplied : Sterile filtered and the the protein was lyophilized from 2X PBS pH7.2( no additives).
Purity : >95% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of FGF-5.
Reconstitution : The lyophilized FGF-5 should be reconstituted in 1X PBS pH7.2 to a concentration of 1ug/ul (1mg/ml). Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4°Cfor up to 1 week or stored at - 20°Cfor future use. For most in-vitro applications, human FGF-5 exerts its biological activity in the concentration range of 0.1 to 20ng/ml.
Note : If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration >50ug/ml.
Reactivity : The biological activity was determined by the does- dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 <1ng/ml, corresponding to a specific activity of >1 X 106 (1 million) units/mg.
Amino Acid Sequence : MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAASL GSQGSGLEQS SFQWSPSGR RTGSLYCRVG IGFHLQIYPDG KVNGSHEANM LSVLEIFAVSQ GIVGIRGVFSN KFLAMSKKGK LHASAKFTDD CKFRERFQEN SYNTYASAIHR TEKTGREWYV ALNKRGKAKR GCSPRVKPQH ISTHFLPRFK QSEQPE LSFTV TVPEK KNPPSP IKSK IPLSAPRKNT NSVKYRLKFRFG
Storage : The lyophilized powder is stable at room temperature but it is best stored desiccated below 0°C. Reconstituted human FGF-5 should be stored in working aliquots at -20℃.
Gene Name : FGF5 fibroblast growth factor 5 [ Homo sapiens ]
Synonyms : FGF5; fibroblast growth factor 5; HBGF-5; Smag-82; heparin-binding growth factor 5; FGF-5; Heparin-binding growth factor 5
Gene ID : 2250
mRNA Refseq : NM_004464
Protein Refseq : NP_004455
MIM : 165190
UniProt ID : P12034
Chromosome Location : 4q21
Pathway : MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton
Function : growth factor activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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