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Recombinant Human Matrix Metallopeptidase 1, Catalytic Domain

Cat.No. : MMP1-148H
Product Overview : Recombinant Human matrix metalloproteinase-1 (MMP-1, fibroblast collagenase, collagenase-1, interstitial collagenase) cloned from human cDNA was expressed inE. coli. The enzyme consists of the catalytic domain of human MMP-1 (residues 106-261, swissprot accession P03956). MW=17.6 kDa.
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  • Gene Information
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Cat. No. : MMP1-148H
Description : Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP"s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.In addition, mechanical force may increase the expression of MMP1 in human periodontal ligament cells.
Source : E.Coli.
Purity : > 95% by SDS-PAGE. The enzyme was observed as a single band migrating at a molecular weight <20 kDa.
Specific Activity : >100U/μg. Activity described as U=100 pmol/min at 25ºC using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.
Usage : Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.
Supplied As : 0.2mg/ml in 20mM Tris, pH 7.2, 10mM CaCl2, 0.1mM ZnCl2, 0.3M NaCl, 0.2M Acetohydroxamic Acid (AHA). The concentration is calculated from the absorbance at 280nm. (e280 = 25440 M-1 cm-1).
Note : Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated to a maximum concentration of 1mM.
Storage : -80ºC. The enzyme is stable at -20ºC for at least 1 week. After initial defrost, aliquot enzyme into individual tubes and refreeze at -80ºC. Avoid repeated freeze/defrost cycles.
Gene Name : MMP1 matrix metallopeptidase 1 (interstitial collagenase) [ Homo sapiens ]
Synonyms : MMP1; matrix metallopeptidase 1 (interstitial collagenase); CLG; CLGN; matrix metalloproteinase 1; fibroblast collagenase; matrix metalloprotease 1; EC 3.4.24.7; Interstitial collagenase; Matrix metalloproteinase-1; MMP-1; Fibroblast collagenase; 22 kDa interstitial collagenase; 27 kDa interstitial collagenase
Gene ID : 4312
mRNA Refseq : NM_001145938
Protein Refseq : NP_001139410
MIM : 120353
UniProt ID : P03956
Chromosome Location : 11q21-q22
Pathway : Bladder cancer; PPAR signaling pathway; Pathways in cancer; Diabetes pathways; Hemostasis; Signaling in Immune system
Function : calcium ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does MMP1 relate to wound healing? 02/09/2023

MMP1 is involved in the remodeling of tissue during wound healing by facilitating the breakdown of damaged extracellular matrix components.

Are there therapeutic implications of targeting MMP1 in cancer treatment? 11/11/2022

Inhibiting MMP1 activity is being explored as a potential therapeutic strategy to impede cancer progression and metastasis.

How does MMP1 contribute to tissue remodeling? 05/07/2020

MMP1 plays a key role in tissue remodeling by degrading collagen, a major component of the extracellular matrix, which is essential for processes like wound healing.

In what diseases is MMP1 overexpressed? 08/21/2017

MMP1 overexpression is associated with various diseases, including rheumatoid arthritis, atherosclerosis, and certain types of cancer.

Can MMP1 be a biomarker for prognosis in cancer patients? 02/18/2017

High MMP1 levels have been correlated with poor prognosis in certain cancer types, making it a potential biomarker for predicting disease outcomes.

Customer Reviews (3)

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Reviews
08/26/2021

    The manufacturer can offer technical support and expertise to guide researchers through the experimental process.

    11/09/2020

      The manufacturer can play a vital role in supporting and optimizing the research process.

      07/20/2020

        This guarantees that the protein is reliable, consistent, and suitable for experiments, reducing variability and enhancing the reproducibility of findings.

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