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Recombinant Human MMP9 protein

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GST(1)
His(19)
N/A(5)
None(2)
T7(1)
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Cat.No.:
MMP9-155H
Product Overview:
Recombinant Human MMP9 catalytic domain(Phe107-Pro449) fused with purification tag at C-terminal was expressed in E. coli.
Description:
Matrix metallopeptidase 9 (MMP-9) belongs to a class of enzymes that belong to the zinc-metalloproteinase family involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, angiogenesis, bone development, wound healing, cell migration, learning and memory. Its also associated with numerous pathological processes, including cancer, immunologic and cardiovascular diseases.
Source:
E. coli
Species:
Human
Form:
Liquid. In 50mM TRIS, pH 7.5, containing 1mM calcium chloride, 300mM sodium chloride, 5μM zinc chloride, 0.1% Brij-35 and 15% glycerol.
Bio-activity:
≥20 U/µg
Molecular Mass:
39 kDa
Purity:
≥95% (SDS-PAGE)
Unit Definition:
One U=100pmol/min at 37 centigrade using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100µM) as substrate.
Applications:
Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
Storage:
Long Term Storage: -80 centigrade. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80 centigrade.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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