Active Recombinant Human MMP12

• Cat.No.: MMP12-157H
• Product Overview: Recombinant Human MMP12 (NP_002417.2) (Gly106-Asn268), fused with a N-terminal Met, was expressed in E.coli.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 10 mM Hepes, 2 mM CaCl2, 250 mM NaCl, pH 7.0, 5 % trehalose and mannitol are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 800 pmoles/min/µg.
• Molecular Mass: The recombinant human MMP12 consists of 164 amino acids and predicts a molecular mass of 18.2 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: Please contact us for more information.
• Purity: > 90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC to -80ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: MMP12 matrix metallopeptidase 12 (macrophage elastase) [ Homo sapiens (human) ]
• Official Symbol: MMP12
• Synonyms: MMP12; matrix metallopeptidase 12 (macrophage elastase); HME; MME; MGC138506; MMP-12; ME; macrophage elastase; macrophage metalloelastase; EC 3.4.24.65; Matrix metalloproteinase-12; matrix etalloproteinase 12; Macrophage elastase; matrix metalloproteinase 12 (macrophage elastase)
• Gene ID: 4321
• mRNA Refseq: NM_002426
• Protein Refseq: NP_002417
• MIM: 601046
• UniProt ID: P39900
• Chromosome Location: 11q22.3
• Pathway: Matrix Metalloproteinases
• Function: calcium ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding; endopeptidase activity