Recombinant Human ACE2, Fc Chimera

Cat. No.

ACE2-239H 

Product Overview

Recombinant Human ACE2 encoding the extracellular domain (Met 1- Ser 740 ) of human ACE2 precursor (NP_068576.1) was expressed with the fused Fc region of human IgG1 at the C-terminus. It was produced in Human Cells (HEK293).

Description

Angiotensin-converting enzyme 2 (ACE2), also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE. ACE2 is identified as a zinc metalloprotease structurally consisting of a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE2 hydrolyzes Ang I and Ang II into inactive Ang (1–9) to Ang (1–7) respectively via its carboxypeptidase activity. A critical characteristic of ACE2 is that its enzymatic activity is not affected by classical ACE inhibitors.

Molecular Mass

The recombinant human ACE2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 961 amino acids and predicts a molecular mass of 110.3 KDa. As a result of glycosylation, the rhACE2/Fc monomer migrates as approximately 145-150 KDa band in SDS-PAGE under reducing conditions.

Source

Human Cells (HEK293).

Predicted N Terminal

Gln 38.

Purity

> 95%, as determined by SDS-PAGE.

Formulation

Supplied as a 0.2μm filtered solution of 100mM Glycine, 10mM NaCl, 50 mM Tris, pH 7.5.

Stability

Samples are stable for up to twelve months from date of receipt -70^oC.

Endotoxin

< 1.0 EU per 1μg cytokine as determined by the LAL method.

Reconstitution

Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Storage

Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted into smaller quantities and be used as soon as possible. Avoid repeated freeze-thaw cycles.

Gene Information

Gene Name

ACE2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Homo sapiens ]

Synonyms

ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; ACEH; DKFZp434A014; angiotensin I converting enzyme; ACE-related carboxypeptidase2; angiotensin converting enzyme-like protein; EC 3.4.15.1; EC 3.4.17.-; Angiotensin-converting enzyme 2; ACEH

GeneID

59272

mRNA Refseq

NM_021804

Protein Refseq

NP_068576

MIM

300335

UniProt ID

Q9BYF1

Chromosome Location

Xp22

Pathway

Renin-angiotensin system

Function

carboxypeptidase activity; chloride ion binding; glycoprotein binding; metal ion binding; metallopeptidase activity; peptidase activity; peptidyl-dipeptidase activity; viral receptor activity; zinc ion binding