Active Recombinant Human ANGPT2 protein, hFc-tagged

• Cat.No.: ANGPT2-200H
• Product Overview: Recombinant Human ANGPT2 protein(NP_001138.1)(Met1-Phe496), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: hFc
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human Angiopoietin-2 at 10 μg/ml (100 μl/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 μg/ml.
• Molecular Mass: The recombinant human Ang2/Fc is a disulfide-linked homodimeric Protein after removal of the signal pep. The reduced monomer consists of 716 amino acids and predicts a molecular mass of 81.6 kDa. As a result of glycosylation, the rhAng2/Fc monomer migrates as approximately 110-115 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Phe496
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Homo sapiens ]
• Official Symbol: ANGPT2
• Synonyms: VASN; vasorin; slit like 2 (Drosophila) , SLITL2; slit-like 2; protein slit-like 2; SLITL2
• Gene ID: 285
• mRNA Refseq: NM_001118887
• Protein Refseq: NP_001112359
• MIM: 601922
• UniProt ID: O15123