Active Recombinant Human BACE1 protein

• Cat.No.: BACE1-255H
• Product Overview: Recombinant Human BACE1 protein(NP_036236.1)(Met1-Thr457) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N/A
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2. Cleavage of ES004 can be measured using excitation and emission wavelength of 320 and 405 nm, respectively. The specific activity is >3.5 pmoles/min/μg.
• Molecular Mass: The recombinant human BACE1 consists of 442 amino acids and has a calculated molecular mass of 49 kDa. As a result of glycosylation, the rhBACE1 migrates as an approximately 65 kDa protein in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Thr457
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BACE1 beta-site APP-cleaving enzyme 1 [ Homo sapiens ]
• Official Symbol: BACE1
• Synonyms: BACE1; beta-site APP-cleaving enzyme 1; BACE, beta site APP cleaving enzyme; beta-secretase 1; asp 2; memapsin-2; APP beta-secretase; aspartyl protease 2; beta-site APP cleaving enzyme 1; beta-secretase 1 precursor variant 1; transmembrane aspartic proteinase Asp2; membrane-associated aspartic protease 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme; ASP2; BACE; HSPC104; FLJ90568; KIAA1149
• Gene ID: 23621
• mRNA Refseq: NM_001207048
• Protein Refseq: NP_001193977
• MIM: 604252
• UniProt ID: P56817