Recombinant Human CA9 protein, His-tagged
Cat.No. : | CA9-256H |
Product Overview : | Recombinant Human CA9 (Gln138-Asp414) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293). |
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Description : | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε). Carbonic anhydrase 9 (CA9/CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9/CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia. |
Source : | HEK293 |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Gln138 |
Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose. |
Molecular Mass : | The protein has a calculated MW of 30.9 kDa. The protein migrates as 36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein length : | Gln138-Asp414 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | CA9 |
Official Symbol : | CA9 |
Synonyms : | CA9; carbonic anhydrase IX; carbonic anhydrase 9; CAIX; carbonic dehydratase; MN; RCC associated protein G250; pMW1; CA-IX; P54/58N; membrane antigen MN; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250 |
Gene ID : | 768 |
mRNA Refseq : | NM_001216 |
Protein Refseq : | NP_001207 |
MIM : | 603179 |
UniProt ID : | Q16790 |
Products Types
◆ Recombinant Protein | ||
CA9-481H | Recombinant Human CA9 Protein, His (Fc)-Avi-tagged | +Inquiry |
Car9-1962M | Recombinant Mouse Car9 Protein, Myc/DDK-tagged | +Inquiry |
CA9-116H | Recombinant Human CA9 Protein, His-tagged | +Inquiry |
CA9-117H | Recombinant Human CA9 Protein, His-tagged | +Inquiry |
CA9-0250H | Recombinant Human CA9 Protein, GST-Tagged | +Inquiry |
◆ Lysates | ||
CA9-3065HCL | Recombinant Human CA9 cell lysate | +Inquiry |
CA9-2189MCL | Recombinant Mouse CA9 cell lysate | +Inquiry |
CA9-1447CCL | Recombinant Canine CA9 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionThe HIF-1 signaling pathway primarily regulates CA9 in response to cellular oxygen levels.
CA9 aids in extracellular acidification, promoting tumor cell survival and invasion in acidic tumor microenvironments.
CA9 expression is upregulated by the hypoxia-inducible factor (HIF) under low oxygen conditions.
Elevated CA9 levels often correlate with poor prognosis in various cancers due to its role in tumor adaptation to hypoxia.
Potential modifications like glycosylation can be identified using techniques like mass spectrometry.
Several inhibitors, like acetazolamide derivatives, have been explored to target CA9 in cancer treatment.
CA9 helps tumors survive in hypoxic conditions by regulating pH through the conversion of CO2 to bicarbonate and protons.
Customer Reviews (3)
Write a reviewExceptional consistency, vital for our research's reliability.
Critical support for our protein analysis and quantification.
Unmatched accuracy in protein identification and sequencing.
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