Active Recombinant Human DPP4 protein

• Cat.No.: DPP4-270H
• Product Overview: Recombinant Human DPP4 protein(Asn 29 - Pro 766 ) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N/A
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >11000 pmol/min/µg.
• Molecular Mass: This protein carries no "tag"The protein has a calculated MW of 85.5 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Asn29-Pro766
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Comparison of the susceptibility of porcine and human dipeptidyl-peptidase IV to inhibition by protein-derived peptides (2015)
  Peptide Array on Cellulose Support-A Screening Tool to Identify Peptides with Dipeptidyl-Peptidase IV Inhibitory Activity within the Sequence of α-Lactalbumin. (2014)

Gene Information

• Gene Name: DPP4 dipeptidyl-peptidase 4 [ Homo sapiens ]
• Official Symbol: DPP4
• Synonyms: DPP4; dipeptidyl-peptidase 4; ADCP2, adenosine deaminase complexing protein 2 , CD26, dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); dipeptidyl peptidase 4; DPPIV; ADCP-2; DPP IV; dipeptidylpeptidase 4; dipeptidyl peptidase IV; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); CD26; ADABP; ADCP2; TP103
• Gene ID: 1803
• mRNA Refseq: NM_001935
• Protein Refseq: NP_001926
• MIM: 102720
• UniProt ID: P27487