Active Recombinant Human CTSS protein(Met1-Ile331), His-tagged

• Cat.No.: CTSS-282H
• Product Overview: Recombinant Human CTSS (NP_004070.3) (Met 1-Ile 331) with a carboxy-terminal polyhistidine tag was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Ile331
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl) acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys (2, 4-dinitrophenyl)-NH2. Cleavage of ES002 can be measured using excitation and emission wavelength at 320 nm and 405 nm, respectively. The specific activity is >300 pmoles/min/μg.(Activation description: The enzyme achieves its activity under acidic pH)
• Molecular Mass: The secreted recombinant human CTSS consists of 326 amino acids with the predicted molecular mass of 37 kDa as estimated by SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CTSS cathepsin S [ Homo sapiens ]
• Official Symbol: CTSS
• Synonyms: CTSS; cathepsin S; MGC3886; FLJ50259
• Gene ID: 1520
• mRNA Refseq: NM_001199739
• Protein Refseq: NP_001186668
• MIM: 116845
• UniProt ID: P25774