Active Recombinant Human DDC protein(Met1-Glu480), His-tagged

• Cat.No.: DDC-284H
• Product Overview: Recombinant full length of Human DDC (NP_000781.1) (Met 1-Glu 480) was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: C-His
• Protein length: Met1-Glu480
• Form: Supplied as sterile 50mM Tris, 100mM NaCl, pH 8, 10% glycerol.
• Bio-activity: Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1000 pmoles/min/μg.
• Molecular Mass: The recombinant human DDC consists of 490 amino acids and predicts a molecular mass of 55 kDa. It migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: Please refer to the hard copy of CoA.

Gene Information

• Gene Name: DDC dopa decarboxylase (aromatic L-amino acid decarboxylase) [ Homo sapiens ]
• Official Symbol: DDC
• Synonyms: DDC; dopa decarboxylase (aromatic L-amino acid decarboxylase); aromatic-L-amino-acid decarboxylase; AADC
• Gene ID: 1644
• mRNA Refseq: NM_000790
• Protein Refseq: NP_000781
• MIM: 107930
• UniProt ID: P20711