Active Recombinant Human GZMB, His tagged
Cat.No. : | GZMB-296H |
Product Overview : | Recombinant Human GZMB proform (NP_004122.1) (Met 1-Tyr 247), fused with a C-terminal polyhistidine tag, was produced in HEK293 Cells. |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its ability to cleave a peptide substrate, tButyloxycaronylAlaAlaAspThioBenzylester (BocAADSBzl),in the presence of 5,5’Dithiobis (2nitrobenzoic acid) (DTNB). The specific activity is >1500 pmol/min/μg. (The enzyme needs to be activated by Recombinant Mouse Active Cathepsin C) |
Molecular Mass : | The recombinant human Granzyme B consists of 240 amino acids and has a calculated molecular mass of 27.2 kDa. As a result of glycosylation, the apparent molecular mass of rhGranzyme B is approximately 35-40 kDa in SDS-PAGE under reducing conditions. |
Protein Length : | Met 1-Tyr 247 |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | ≥ 97 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | GZMB granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) [ Homo sapiens ] |
Official Symbol : | GZMB |
Synonyms : | GZMB; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); CSPB, CTLA1; granzyme B; cathepsin G like 1; CCPI; CGL 1; CGL1; CSP B; CTSGL1; cytotoxic serine protease B; fragmentin 2; HLP; SECT; T cell serine protease 1 3E; C11; CTLA-1; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic T-lymphocyte proteinase 2; CSPB; CGL-1; CSP-B; CTLA1; |
Gene ID : | 3002 |
mRNA Refseq : | NM_004131 |
Protein Refseq : | NP_004122 |
MIM : | 123910 |
UniProt ID : | P10144 |
Products Types
◆ Recombinant Protein | ||
Gzmb-273G | Active Recombinant Mouse Gzmb Protein (227 aa) | +Inquiry |
GZMB-2425R | Recombinant Rat GZMB Protein, His (Fc)-Avi-tagged | +Inquiry |
Gzmb-4520M | Recombinant Mouse Gzmb Protein | +Inquiry |
Gzmb-2516M | Recombinant Mouse Gzmb Protein | +Inquiry |
Gzmb-070M | Active Recombinant Mouse Gzmb Protein | +Inquiry |
◆ Lysates | ||
GZMB-2944HCL | Recombinant Human GZMB cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0403 | GZMB Activity Fluorometric Assay Kit | +Inquiry |
Kit-0404 | GZMB Assay/Inhibitor Screening Fluorometric Kit | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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