Active Recombinant Mouse Cxadr protein(Met1-Gly237), His&hFc-tagged

• Cat.No.: Cxadr-676M
• Product Overview: Recombinant Mouse CXADR (NP_001020363.1)(Met 1-Gly 237) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His&hFc
• Protein length: Met1-Gly237
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 5 x 10E4 cells/well are added to CXADR-coated plates (4 μg/ml and 100 μl/well), approximately 20%-40% will adhere specifically after 60 minutes at 37°C.
• Molecular Mass: The recombinant mouse CXADR/Fc is a disulfide-linked homodimer. The reduced monomer consists of 466 amino acids and has a predicted molecular mass of 52 kDa. As a result of glycosylation, the apparent molecular mass of rm CXADR/Fc monomer migRates with an apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cxadr coxsackie virus and adenovirus receptor [ Mus musculus ]
• Official Symbol: Cxadr
• Synonyms: CXADR; coxsackie virus and adenovirus receptor; coxsackievirus and adenovirus receptor homolog; CAR; MCAR; MCVADR; AU016810; AW553441; 2610206D03Rik
• Gene ID: 13052
• mRNA Refseq: NM_001025192
• Protein Refseq: NP_001020363