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Active Recombinant Human NBL1 protein(Met1-Asp180), His-tagged

Cat.No. : NBL1-682H
Product Overview : Recombinant Human DAN precursor (NP_005371.1) (Met 1-Asp 180) was expressed in HEK293 with a C-terminal polyhistidine tag.
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Source : HEK293
Species : Human
Tag : C-His
Protein length : Met1-Asp180
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 μg/ml in the presence of 25 ng/mL of recombinant human BMP4.
Molecular Mass : The recombinant human DAN consists of 176 amino acids and has a calculated molecular mass of 19.3 kDa. As a result of glycosylation, rh DAN migrates as an approximately 27 kDa protein in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : > 98 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : NBL1 neuroblastoma, suppression of tumorigenicity 1 [ Homo sapiens ]
Official Symbol : NBL1
Synonyms : NBL1; neuroblastoma, suppression of tumorigenicity 1; neuroblastoma suppressor of tumorigenicity 1; D1S1733E; DAN; DAND1; differential screening selected gene aberrant in neuroblastoma; NB; neuroblastoma candidate region; suppression of tumorigenicity 1; NO3; DAN domain family member 1; differential screening-selected gene aberrant in neuroblastoma; neuroblastoma candidate region, suppression of tumorigenicity 1; MGC8972;
Gene ID : 4681
mRNA Refseq : NM_001204084
Protein Refseq : NP_001191013
MIM : 600613
UniProt ID : P41271

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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