Recombinant RSV-F F extracellular domain (AAB59858.1) (Met 1-Thr 529), fused with a polyhistidine tag at the C-terminus, was produced in Baculovirus-Insect cells.
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol
The secreted recombinant human RSV-F comprises 518 amino acids with a predicted molecular mass of 58 kDa. The RSV F0 precursor protein is cleaved into the disulfide-linked F1 and F2 subunits. As a result of glycosylation, the apparent molecular mass of the protein is approximately 45-55 kDa and 18 kDa in SDS-PAGE under reducing conditions, corresponding to the two subunits respectively.
< 1.0 eu per μg of the protein as determined by the LAL method.
Samples are stable for up to twelve months from date of receipt at -70oC.
Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.