Recombinant Human PAPPA2 protein(Ser234-Cys1396), His-tagged

• Cat.No.: PAPPA2-821H
• Product Overview: Recombinant Human PAPPA2(NP_064714.2)(Ser 234-Cys 1396) was expressed in HEK293, with a carboxy-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Ser234-Cys1396
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human PAPPA2 consists of 1174 amino acids with the predicted molecular mass of 131 kDa. As a result of glycosylation, rhPAPPA2 migrates as an approximately 170-180 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PAPPA2 pappalysin 2 [ Homo sapiens ]
• Official Symbol: PAPPA2
• Synonyms: PAPPA2; pappalysin 2; PLAC3, placenta specific 3; pappalysin-2; PAPP A2; PAPPE; PAPP-E; placenta-specific 3; pregnancy-associated plasma protein E1; pregnancy-associated plasma preproprotein-A2; PLAC3; PAPP-A2
• Gene ID: 60676
• mRNA Refseq: NM_020318
• Protein Refseq: NP_064714
• UniProt ID: Q9BXP8