Active Recombinant Human PDGFC protein(Val235-Gly345), hFc-tagged

• Cat.No.: PDGFC-206H
• Product Overview: Recombinant Human PDGFC (NP_057289.1) (Val 235-Gly 345) was expressed in HEK293 with the fused Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N-hFc
• Protein length: Val235-Gly345
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell prolifeRation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.5-3 μg/mL.
• Molecular Mass: The recombinant human PDGFC/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 348 amino acids and predicts a molecular mass of 39 kDa. As a result of glycosylation, rh PDGFC/Fc monomer is approximately 45 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDGFC platelet derived growth factor C [ Homo sapiens ]
• Official Symbol: PDGFC
• Synonyms: PDGFC; platelet derived growth factor C; platelet-derived growth factor C; fallotein; SCDGF; PDGF-C; VEGF-E; spinal cord-derived growth factor; secretory growth factor-like protein; FALLOTEIN
• Gene ID: 56034
• mRNA Refseq: NM_016205
• Protein Refseq: NP_057289
• MIM: 608452
• UniProt ID: Q9NRA1