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Active Recombinant Human POSTN protein(Met1-Gln781), His-tagged

Cat.No. : POSTN-820H
Product Overview : Recombinant Human POSTN isoform 3 (NP_001129407.1) corresponding to amino acid (Met 1-Gln 781) was expressed in HEK293 with a C-terminal polyhistidine tag.
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Source : HEK293
Species : Human
Tag : C-His
Protein length : Met1-Gln781
Form : Lyophilized from sterile PBS, 5% Trehalose, pH 7.0 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When cells are added to POSTN-His coated plates (10μg/mL, 100μL/well), approximately >60% will adhere specifically after 30 minutes at 37°C.
Molecular Mass : The recombinant human POSTN consists of 771 amino acids and has a predicted molecular mass of 86.4 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 66-80 kDa due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : POSTN periostin, osteoblast specific factor [ Homo sapiens ]
Official Symbol : POSTN
Synonyms : POSTN; periostin, osteoblast specific factor; periostin; OSF 2; PN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; osteoblast-specific factor 2; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like); OSF2; OSF-2; PDLPOSTN; RP11-412K4.1; MGC119510; MGC119511;
Gene ID : 10631
mRNA Refseq : NM_001135934
Protein Refseq : NP_001129406
MIM : 608777
UniProt ID : Q15063

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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