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Active Recombinant Human SFRP4 protein, His-tagged

Cat.No. : SFRP4-864H
Product Overview : Recombinant Human SFRP4 protein(NP_003005.2)(Met1-Val346), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells.The ED50 for this effect is typically 1-6 μg/mL in the presence of 20 ng/mL of Wnt3a.
Molecular Mass : The recombinant human sFRP4 consists of 336 amino acids after removal of the signal peptide and has a predicted molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 55-60 kDa due to glycosylation.
Protein Length : Met1-Val346
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : SFRP4 secreted frizzled-related protein 4 [ Homo sapiens ]
Official Symbol : SFRP4
Synonyms : SFRP4; secreted frizzled-related protein 4; FRP 4; FRPHE; frpHE; sFRP-4; frizzled protein, human endometrium; FRP-4; MGC26498;
Gene ID : 6424
mRNA Refseq : NM_003014
Protein Refseq : NP_003005
MIM : 606570
UniProt ID : Q6FHJ7

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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