"VWF" Related Products

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Recombinant Human VWF, His tagged

Cat.No.: VWF-894H
Product Overview: Recombinant Human VWF pro form (NP_000543.2) (Met 1-Lys 2813), fused with a C-terminal polyhistidine tag, was produced in CHO Cells.
Source: CHO Cells
Species: Human
Tag: His
Form: Lyophilized from sterile PBS, pH 7.4
Molecular Mass: The secreted recombinant human vWF consists of 2802 amino acids after removal of the signal peptide and has a predicted molecular mass of 308 kDa. Purified rhvWf exists as both the pro form with the pro peptide (307 kDa) and the mature form (226 kDa), which migrates as doublets with apparent molecular mass of 260 and 350 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.
Gene Name: VWF von Willebrand factor [ Homo sapiens ]
Official Symbol: VWF
Gene ID: 7450
mRNA Refseq: NM_000552
Protein Refseq: NP_000543
MIM: 613160
UniProt ID: P04275

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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