Recombinant Human Fms-Related Tyrosine Kinase 4

• Cat.No.: FLT4-587H
• Product Overview: Recombinant human soluble Vascular Endothelial Growth Factor Receptor-3 (sVEGFR-3/FLT-4) was fused with a carboxy -terminal 6X histidine-tag. The recombinant mature sVEGFR-3/FLT-4 is a glycosylated monomeric protein. The sVEGFR-3/FLT -4 monomers have a mass of approximately 120 kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Glu774). It is produced inInsect cells.

Specification

• Cat. No.: FLT4-587H
• Description: All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The FLT -4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4.
• Source: Insect cells.
• Subunit: Monomeric glycoprotein.
• Purity: > 90%, by SDS-PAGE and visualised by silver stain.
• Reconstitution: The lyophilised sVEGFR-3/FLT-4 is soluble in water and most aqueous buffers. The lyophilised sVEGFR-3/FLT-4 should be reconstituted in PBS or medium to a concentration not lower than 100 µg/ml.
• Specific Activity: Measured by its ability to bind recombinant rat VEGF-C in a functional solid phase binding assay. Immobilised recombinant human sVEGFR-3/FLT-4 at 5 µg/ml can bind recombinant rat VEGF-C in a linear range of 8-500 ng/ml.
• Endotoxin level: < 0.1 ng per mg of sVEGFR-3.
• Stability: Lyophilised samples are stable for greater than six months at -20°C to -70°C. Reconstituted sVEGFR-3/FLT -4 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: FLT4 fms-related tyrosine kinase 4 [ Homo sapiens ]
• Synonyms: FLT4; fms-related tyrosine kinase 4; PCL; FLT41; LMPH1A; VEGFR3; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; vascular endothelial growth factor receptor 3; EC 2.7.10.1
• Gene ID: 2324
• mRNA Refseq: NM_002020
• Protein Refseq: NP_002011
• MIM: 136352
• UniProt ID: P35916
• Chromosome Location: 5q34-q35
• Pathway: Cytokine-cytokine receptor interaction; Focal adhesion; Signaling by VEGF
• Function: ATP binding; growth factor binding; receptor activity; transferase activity; nucleotide binding; vascular endothelial growth factor receptor activity