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Recombinant Human TNFRSF1B

Cat.No. : NFRSF1B-590H
Product Overview : Recombinant human sTNFRII is an 18.9 kDa protein (174 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFR1 protein. It is produced inInsect cells.
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Cat. No. : TNFRSF1B-590H
Description : TNFRII is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF-alpha but manifests a lower affinity to TNF-beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. Soluble TNFRII is capable of inhibiting TNF-alpha induced activities by acting as a decoy receptor.
Source : Insect cells.
Amino Acid Sequence : MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP.
Supplied : Lyophilized protein from 10mM Tris, pH8.0 + 50mM NaCl.
Note : If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Purity : >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human sTNFR2.
Reconstitution : The lyophilized sTNFR2 is soluble in water and most aqueous buffers. The lyophilized powder should first be quick spun in vial, then reconstituted in water to a concentration of 1ug/ul. Allow to set at least 30 minutes at 4℃. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at -20℃ for future use.
Reactivity : Determined by its inhibitory effect of the TNF- alpha mediated cytoxicity in murine L929 cells. The Ed50for this effectin the presence of 0.25ng/ml of recombinant human TNF-Ala is 0.125ug/ml.
Storage : The lyophilized powder, though stable at room temperature, is best stored dessicated below 0℃. Reconstituted sTNFR2 should be stored in working aliquots at -20℃. Avoid Frequent Freeze-Thaw Cycles.
Gene Name : TNFRSF1B tumor necrosis factor receptor superfamily, member 1B [ Homo sapiens ]
Synonyms : TNFRSF1B; tumor necrosis factor receptor superfamily, member 1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II; p75 TNF receptor; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2
Gene ID : 7133
mRNA Refseq : NM_001066
Protein Refseq : NP_001057
MIM : 191191
UniProt ID : P20333
Chromosome Location : 1p36.22
Pathway : Adipocytokine signaling pathway; Amyotrophic lateral sclerosis (ALS); Cytokine-cytokine receptor interaction
Function : protein binding; receptor activity; tumor necrosis factor receptor activity

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What is the structural classification of TNFRSF1B, and how does its extracellular domain contribute to ligand binding and signaling? 01/29/2022

TNFRSF1B is a type I transmembrane protein with an extracellular domain containing cysteine-rich repeats. This domain is crucial for ligand binding, receptor trimerization, and subsequent signaling.

How does TNFRSF1B contribute to the regulation of inflammation, and what are the implications of its dysregulation in inflammatory diseases? 10/26/2021

TNFRSF1B regulates inflammation by influencing the expression of inflammatory mediators. Dysregulation is associated with inflammatory diseases, highlighting its role in immune system homeostasis.

What is the role of TNFRSF1B in apoptosis, and how does its signaling pathway intersect with both extrinsic and intrinsic apoptotic pathways? 02/18/2020

TNFRSF1B plays a role in apoptosis by influencing extrinsic apoptotic pathways. Its signaling can intersect with intrinsic pathways through crosstalk with mitochondria and pro-apoptotic proteins.

Can you discuss the post-translational modifications of TNFRSF1B, such as phosphorylation, and their impact on receptor signaling and cellular responses? 05/09/2019

Post-translational modifications, including phosphorylation, modulate TNFRSF1B signaling. Phosphorylation events influence downstream signaling cascades and cellular responses to ligand binding.

Can you elaborate on the ligands that bind to TNFRSF1B, particularly tumor necrosis factor (TNF)-β, and the conformational changes that occur upon ligand binding? 09/05/2018

TNFRSF1B binds specifically to TNF-β. Ligand binding induces conformational changes in the receptor, leading to the recruitment of signaling molecules and the initiation of downstream pathways.

Customer Reviews (3)

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Reviews
01/09/2020

    Fast shipping and excellent service. This product is a lab essential.

    05/16/2019

      Quality meets expectations. The consistency is perfect for our experiments.

      06/26/2018

        Customer-centric service and high-quality products. Our experiments have benefited tremendously.

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