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Recombinant Human Interleukin 12B

Cat.No. : IL12B-177H
Product Overview : Recombinant human IL-12 p40 is a 40 kDa protein containing 306 amino acid residues. It was expressed in E.Coli.
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Cat. No. : IL12B -177H
Description : Interleukin-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23.
Source : E.Coli.
Purity : > 95% by SDS-PAGE and HPLC analysis.
Endotoxin Level : < 0.1 ng per μg of IL-12p40.
Stabilizer : None.
Formulation : Lyophilized.
Biological Activity : Not available.
Reconstitution : The human IL-12p40 should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.
Stability : The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted human IL-12p40 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Gene Name : IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
Synonyms : OTTHUMP00000160820; CLMF; CLMF2; IL-12B; NKSF; NKSF2; CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 subunit p40; IL12, subunit p40; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); IL12B
Gene ID : 3593
mRNA Refseq : NM_002187
Protein Refseq : NP_002178
MIM : 161561
UniProt ID : P29460
Chromosome Location : 5q31.1-q33.1
Pathway : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus
Function : contributes_to cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-12 alpha subunit binding; interleukin-12 receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; signal transducer activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation? 05/12/2021

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity? 05/02/2021

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells? 10/30/2020

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation? 05/25/2020

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies? 04/30/2020

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners? 04/12/2018

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function? 12/03/2017

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets? 10/31/2017

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation? 09/29/2017

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context? 08/22/2017

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.

Customer Reviews (3)

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Reviews
04/08/2020

    Good for cytokine assays.

    01/06/2020

      Effective in Th2 cell differentiation.

      01/12/2019

        High yield in expression.

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