Recombinant Human TNFRSF4

• Cat.No.: TNFRSF4-967H
• Product Overview: Recombinant human OX40L produced inInsect cellsis a glycosylated 133 amino acid protein corresponding to the extracellular TNF homologous domain of the full length transmembrane protein. It migrates with an apparent molecular mass of 15.5 – 25.0 kDa on SDS-PAGE.

Specification

• Cat. No.: TNFRSF4-967H
• Description: OX40L, a member of the TNF superfamily of structurally related proteins, exists primarily as a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells (APCs), such as dendritic cells and activated B-cells, and also on various other cells such as vascular endothelial cells, mast cells, and natural killer cells. OX40L signals specifically through the OX40 receptor, which is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. OX40/OX40L functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNγ. OX40/OX40L signaling plays an important role in immuno-regulatory communication, enabling the immune system to distinguish between “friend vs. foe” during activation; a mechanism typically termed immuno-tolerance.
• Source: Insect cells.
• Purity: > 97%, by SDS-PAGE and HPLC analysis.
• Endotoxin Level: < 0.1 ng perugof sOX40 Ligand.
• Stabilizer: None.
• Formulation: Lyophilized.
• Biological Activity: Determined by its ability to stimulate IL-8 production by human PBMC using a concentration range of 30-100 ng/ml. Note: Results may vary with PBMC donors.
• Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -20℃. Reconstituted sOX40 Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at-20℃. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: TNFRSF4 tumor necrosis factor receptor superfamily, member 4 [ Homo sapiens ]
• Synonyms: tumor necrosis factor receptor superfamily, member 4; OX40; ACT35; CD134; TXGP1L; TNFRSF4; tax-transcriptionally activated glycoprotein 1 receptor; lymphoid activation antigene ACT35; OX40 cell surface antigen; OX40 homologue; CD134 antigen; ATC35 antigen; OX40 antigen; OTTHUMP00000001522; ACT35 antigen; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor
• Gene ID: 7293
• mRNA Refseq: NM_003327
• Protein Refseq: NP_003318
• MIM: 600315
• UniProt ID: P43489
• Chromosome Location: 1p36
• Pathway: Cytokine-cytokine receptor interaction
• Function: protein binding; receptor activity; tumor necrosis factor receptor activity