Recombinant Human Fms-related Tyrosine Kinase 1, Fc Chimera
Cat.No. : | FLT1-979H |
Product Overview : | Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 (sVEGFR-1D1-7) produced inInsect cellswas fused with the Fc part of human IgG1. The recombinant mature sVEGFR-1D1-7/Fc is a disulfide-linked homodimeric protein. The sVEGFR-1D1-7/Fc monomers have a mass of approximately 130 kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Thr751), which contain all the information necessary for high affinity ligand binding. |
- Specification
- Gene Information
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Cat. No. : | FLT1-979H |
Description : | Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signalling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation. Differential splicing of the flt-1 gene leads to the formation of a secreted, soluble variant of VEGFR-1 (sVEGFR-1). No naturally occurring, secreted forms of VEGFR-2 have so far been reported. The binding of VEGF165 to VEGFR-2 is dependent on heparin. |
Source : | Insect cells. |
Purity : | > 95%, by SDS-PAGE and visualised by silver stain. |
Endotoxin level : | < 0.1 ng per μg of sVEGFR-1. |
Stabilizer : | None. |
Formulation : | Lyophilized. |
Buffer : | PBS; pH 7.4. |
Biologically Activity : | The activity of sVEGFR-1/Fc was determined by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells. The ED50 for this effect is typically 10-30 ng/ml. |
Reconstitution : | The lyophilised sVEGFR-1/Fc is soluble in water and most aqueous buffers. The lyophilised sVEGFR-1/Fc should be reconstituted in PBS or medium to a concentration not lower than 50 μg/ml. |
Stability : | Lyophilised samples are stable for greater than six months at –20°C to –70°C. Reconstituted sVEGFR-1/Fc should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles! |
Gene Name : | FLT1 fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) [ Homo sapiens ] |
Synonyms : | FLT1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FLT; VEGFR1; vascular endothelial growth factor receptor 1; EC 2.7.10.1; VEGFR-1; Vascular permeability factor receptor; Flt-1; Tyrosine-protein kinase receptor FLT; Tyrosine-protein kinase FRT; Fms-like tyrosine kinase 1; FRT |
Gene ID : | 2321 |
mRNA Refseq : | NM_001159920 |
Protein Refseq : | NP_001153392 |
MIM : | 165070 |
UniProt ID : | P17948 |
Chromosome Location : | 13q12 |
Pathway : | Cytokine-cytokine receptor interaction; Endocytosis; Focal adhesion; Signaling by VEGF |
Function : | ATP binding; growth factor binding; identical protein binding; nucleotide binding; receptor activity; transferase activity; vascular endothelial growth factor receptor activity |
Products Types
◆ Recombinant Protein | ||
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Flt1-49M | Recombinant Mouse Flt1 Protein, His (Fc)-Avi-tagged | +Inquiry |
FLT1-3810H | Active Recombinant Human FLT1 Protein, His-Avi-tagged, Biotinylated | +Inquiry |
FLT1-4369H | Recombinant Human FLT1 Protein, GST-tagged | +Inquiry |
◆ Lysates | ||
FLT1-1207RCL | Recombinant Rat FLT1 cell lysate | +Inquiry |
FLT1-1909HCL | Recombinant Human FLT1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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