Active Recombinant Human Breast Cancer 1, Early Onset

• Cat.No.: BRCA1-1018H

Specification

• Cat. No.: BRCA1-1018H
• Description: The human BRCA1, the product of breast/ovary cancer gene 1, is a hyperphosphorylated protein functioning as a tumor suppressor involved in both transcription regulation and DNA repair. BRCA1 associates with RAD51 and has shown to be required for transcription- coupled repair of DNA damage as well as for the repair of chromosomal double-strand breaks. Association with a human SWI/SNF-related complex has recently suggested a potential role of BRCA1 in linking chromatin remodeling to breast cancer. Dysfunction of BRCA sensitizes cells to the inhibition of PARP [Poly(ADP-ribose) polymerase] enzymatic activity, resulting in chromosomal instability, cell cycle arrest, and subsequent apoptosis. The wild type BRCA1 (1-1863 residues) is expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography. Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase, and RNase activity.
• Source: Sf9 insect cells.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
• Purity: > 95% by SDS-PAGE.
• Activity: 1 ng is sufficient for a gel mobility shift assay in a 20 µl reaction, 50 ng are sufficient for reconstituted transcription assays and 100 ng are sufficient for a protein-protein interaction assay.
• Application: Recombinant BRCA1 can be used 1) for in vitro function studies including transcription and DNA repair 2) for protein-protein interaction assay 3) for cell growth assay.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles

Gene Information

• Gene Name: BRCA1 breast cancer 1, early onset [ Homo sapiens ]
• Synonyms: BRCA1; breast cancer 1, early onset; IRIS; PSCP; BRCAI; BRCC1; RNF53; BROVCA1; RING finger protein 53; BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; EC 6.3.2.-
• Gene ID: 672
• mRNA Refseq: NM_007294
• Protein Refseq: NP_009225
• MIM: 113705
• UniProt ID: P38398
• Chromosome Location: 17q21-q24
• Pathway: Ubiquitin mediated proteolysis
• Function: DNA binding; androgen receptor binding; enzyme binding; identical protein binding; ligase activity; metal ion binding; transcription coactivator activity; tubulin binding; ubiquitin-protein ligase activity; zinc ion binding