Active Recombinant Human Interferon, Beta 1, Fibroblast

• Cat.No.: IFNB1-35H

Specification

• Cat. No.: IFNB1-35H
• Description: Interferon β is one of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-β can be induced by common inducers of interferons, including viruses, double-stranded RNA, and microorganisms. It is induced also by some cytokines such as TNF and IL-1. Recombinant human IFN-β 1b produced inE. coliis a single, non-glycosylated form of human Interferon β1b polypeptide chain containing 165 amino acids and having a molecular mass of 18.511 kDa. The Interferon β gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17. IFN- 1b is purified by proprietary chromatographic techniques.
• Source: E. coli.
• Endotoxin: Less than 0.1 ng/μg (IEU/μg) of IFN-β.
• Purity: ≥98% by SDS-PAGE, RP-HPLC and FPLC.
• Form: Lyophilized.
• Solubility: It is recommended to reconstitute the lyophilized IFN-β in sterile bidest H₂O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
• Activity: EC₅₀: 3.2 x 10⁷IU/mg, determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus).
• Storage: Quality guaranteed for 12 months. Store at -20°C.Avoid freeze / thaw cycles.
• Usage: For in vitro use only.

Gene Information

• Gene Name: IFNB1 interferon, beta 1, fibroblast [ Homo sapiens ]
• Synonyms: IFNB1; Interferon, beta 1, fibroblast; IFB; IFF; IFNB; MGC96956; Interferon beta; Leukocyte interferon; B cell interferon; Type I interferon; IFNB1; IFN-b 1a; IFN-beta; OTTHUMP00000021131; Fibroblast interferon
• Gene ID: 3456
• mRNA Refseq: NM_002176
• Protein Refseq: NP_002167
• MIM: 147640
• UniProt ID: P01574
• Chromosome Location: 9p21
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; Toll-like receptor signaling pathway
• Function: cytokine activity; interferon-alpha/beta receptor binding; transcription corepressor activity