Recombinant Human MLLT4, Ras-binding Domain

• Cat.No.: MLLT4-1011H

Specification

• Cat. No.: MLLT4-1011H
• Description: The amino terminus of Af6 was identified as the Rasbinding site. Additionally to the Ras-binding Af6 also binds Rap. ZO-1, a protein involved in the formation of tight junctions, also binds to Af6 close to the amino terminus thereby competing with Ras binding. These data suggest a participation of Af6 in the regulation of cell-cell contacts via a Ras-modulated interaction with ZO-1. In contrast to the well characterized effectors Raf, RalGEF, and PI(3)kinase, Af6 is a protein with no enzymatic function. It seems to serve as a scaffolding component for protein complexes. This would represent a new type of Ras effector because Ras binding in this case does not lead to the activation of the enzymatic activity of the effector.
• Source: E. coli.
• Form: Liquid. Supplied in 25 mM HEPES pH 7.5, 50 mM NaCl, 1 mM EDTA and 50% glycerol.
• Purity: > 95% by SDS-PAGE.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at -20°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: MLLT4 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 [ Homo sapiens ]
• Synonyms: MLLT4; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4; AF6; AF-6; AFADIN; FLJ34371; RP3-431P23.3; myeloid/lymphoid or mixed-lineage leukemia, translocated to, 4;Afadin; Protein AF-6;OTTHUMP00000017632; myeloid/lymphoid or mixed-lineage leukemia (trithorax(Drosophila) homolog); translocated to, 4
• Gene ID: 4301
• mRNA Refseq: NM_001040000
• Protein Refseq: NP_001035089
• MIM: 159559
• UniProt ID: P55196
• Chromosome Location: 6q27
• Pathway: Adherens junction; Leukocyte transendothelial migration; Tight junction
• Function: protein C-terminus binding