Cat. No. : |
SFRS1-1014H |
Description : |
ASF or called SF2, a member of SR protein family, is an essential pre-mRNA splicing factor required for both single and alternative splicing. Phosphorylation on serine residues located within the SR domain directly regulates ASF/SF2 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, such as U1-70K, U2AF and other SR proteins, ASF/SF2 also directly or indirectly interacts with HIV regulatory protein Rev, the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, and numerous transcription factors, thereby suggesting a potential role of ASF/SF2 in coordinating of transcription and pre-mRNA splicing. The human ASF/SF2 wild type protein (residues 1-248) was expressed in E. coli and purified by an affinity column. |
Source : |
E. coli. |
Form : |
Liquid. Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol. |
Purity : |
>95% by SDS-PAGE. |
Activity : |
1 ng is sufficient for a gel mobility shift assay in a 20 μl reaction and 100 ng are sufficient for a protein-protein interaction assay. |
Application : |
Recombinant ASF/SF2 protein can be used 1) for in vitro function studies including pre-mRNA splicing, cross linking and other RNA binding assays.2) for protein-protein interaction assay, and 3) for cell growth and proliferation assays. |
Usage : |
For in vitro use only. |
Storage : |
Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles. |