Recombinant Human Perilipin 2, His-tagged

• Cat.No.: PLIN2-904H
• Product Overview: Recombinant Human Perilipin 2 encoding the human adipose related protein (Met¹- His⁴³⁷) was recombined with his tag at the N-Terminus. That was expressed inE. Coli, and purified by Ni-NTA column.MW=48.1KDa.

Specification

• Cat. No.: PLIN2-904H
• Description: Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation.
• Source: E. Coli.
• Sequence: Human adipose differentiation related protein (Met¹-His⁴³⁷)1MASVAVDPQP SVVTRVVNLP LVSSTYDLMS SAYLSTKDQY PYLKSVCEMA ENGVKTITSV AMTSALPIIQ KLEPQIAVAN TYACKGLDRI EERLPILNQP STQIVANAKG AVTGAKDAVT TTVTGAKDSV ASTITGVMDK TKGAVTGSVE KTKSVVSGSI NTVLGSRMMQ LVSSGVENAL TKSELLVEQY LPLTEEELEK EAKKVEGFDL VQKPSYYVRL GSLSTKLHSR AYQQALSRVK EAKQKSQQTI SQLHSTVHLI EFARKNVYSA NQKIQDAQDK LYLSWVEWKR SIGYDDTDES HCAEHIESRT LAIARNLTQQ LQTTCHTLLS NIQGVPQNIQ DQAKHMGVMA GDIYSVFRNA ASFKEVSDSL LTSSKGQLQK MKESLDDVMD YLVNNTPLNW LVGPFYPQLT ESQNAQDQGA EMDKSSQETQ RSEHKTH 437.
• Purity: >95% in SDS gel.
• Formulation: Lyophilized 100 μg human Perilipin 2 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
• Application: Cell biology, ELISA.
• Reconstitution: Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
• Storage: Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
• Publication:
  Aromatic Functionality of Target Proteins Influences Monomer Selection for Creating Artificial Antibodies on Plasmonic Biosensors. (2016)

Gene Information

• Gene Name: PLIN2 perilipin 2 [ Homo sapiens ]
• Synonyms: ADFP; ADRP; PLIN2; adipose differentiation-related protein; adipophilin; MGC10598; Adipose differentiation-related protein; Adipophilin; Perilipin 2
• Gene ID: 123
• mRNA Refseq: NM_001122
• Protein Refseq: NP_001113
• MIM: 103195
• UniProt ID: Q99541
• Chromosome Location: 9p22.1
• Pathway: Metabolism of lipids and lipoproteins
• Function: protein binding