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Recombinant Human LGALS3, His-tagged

Cat.No. : LGALS3-222H
Product Overview : Recombinant Human LGALS3 encoding the human Galectin 3 (Met1-Ile250P) was recombined with his tag at the N Terminus. That was expressed inE.Coli,and purified by Ni-NTA Column. MW=26.2 KD.
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Cat. No. : LGALS3-222H
Description : Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galactin-3 regulates a number of biological processes, including embryogenesis, inflammatory responses, cell progression and metastasis. Galectin-3 is normally expressed in epithelia of a variety of tissues, including colon and endometrium, and in various inflammatory cells, including macrophages.
Source : E. Coli.
Sequence : Human Galectin-3 (Met1- Ile250P)1MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI 250.
Purity : >95% in SDS gel.
Formulation : Lyophilized 100 μg human Galectin 3 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
Application : Cell biology, ELISA.
Reconstitution : Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
Storage : Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
Gene Name : LGALS3 lectin, galactoside-binding, soluble, 3 [ Canis lupus familiaris ]
Synonyms : LGALS3;lectin, galactoside-binding, soluble, 3; Galectin-3; beta-galactosides-binding lectin; lectin, galactoside-binding, soluble, 3 (galectin 3)
Gene ID : 404021
UniProt ID : P38486

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Are there any diagnostic or prognostic implications of LGALS3 in cancer? 02/23/2023

 LGALS3 has been investigated as a potential diagnostic and prognostic marker in various types of cancer. Its expression levels have been correlated with tumor stage, grade, and patient survival. LGALS3 can be detected in blood, urine, and tissue samples, making it a promising biomarker for non-invasive cancer detection and monitoring. Additionally, LGALS3 expression patterns, subcellular localization, and glycosylation status have been associated with specific cancer subtypes and clinical outcomes, providing insights into disease progression and patient prognosis.

How is the expression of LGALS3 regulated in different tissues and under different conditions? 05/08/2021

The expression of LGALS3 is regulated at multiple levels, including transcriptional, post-transcriptional, and post-translational regulation. Transcription factors, such as Sp1, AP-1, and NF-κB, bind to the promoter region of the LGALS3 gene and regulate its expression. Epigenetic modifications, including DNA methylation and histone acetylation, can also influence LGALS3 expression. Additionally, microRNAs and RNA-binding proteins can regulate the stability and translation of LGALS3 mRNA. Moreover, various extracellular signals, such as cytokines and growth factors, can modulate LGALS3 expression in a tissue-specific and context-dependent manner.

What is the role of the protein LGALS3 in cellular processes? 11/11/2020

LGALS3, also known as galectin-3, is a multifunctional protein involved in various cellular processes. It plays a role in cell adhesion, migration, apoptosis, immune response, and inflammation. LGALS3 can interact with glycosylated proteins and lipids, modulating their functions and signaling pathways. It also acts as a receptor for extracellular matrix proteins and plays a role in tissue remodeling. Additionally, LGALS3 has been implicated in cancer progression and metastasis, as well as in the regulation of fibrosis and wound healing.

Are there therapeutic implications of targeting LGALS3 in cancer treatment? 06/12/2020

Targeting LGALS3 has emerged as a potential therapeutic strategy in cancer treatment. Inhibition of LGALS3 expression or activity can impede tumor cell survival, migration, and invasion. Additionally, blocking LGALS3-mediated immune evasion mechanisms could enhance anti-tumor immune responses. Various approaches, such as small molecule inhibitors, antibodies, and gene silencing techniques, have been explored to target LGALS3 in preclinical and clinical studies. However, further research is needed to optimize these strategies and evaluate their efficacy and safety in cancer patients.

How does LGALS3 contribute to immune response and inflammation? 12/17/2019

LGALS3 plays a critical role in immune response and inflammation. It can modulate the activation and function of immune cells, including macrophages, dendritic cells, and T cells. LGALS3 can promote cell adhesion and migration, facilitating immune cell recruitment to sites of inflammation. It also regulates the production of pro-inflammatory cytokines and chemokines, and can act as a chemoattractant for immune cells. Additionally, LGALS3 can modulate the clearance of apoptotic cells and regulate the resolution of inflammation.

What are the functional domains and motifs present in LGALS3? 09/16/2018

LGALS3 contains a conserved carbohydrate recognition domain (CRD) that allows it to bind to glycosylated proteins and lipids. The CRD is responsible for LGALS3's interaction with specific glycan structures, mediating its diverse functions. LGALS3 also has a N-terminal domain involved in protein-protein interactions and a C-terminal domain that regulates its oligomerization. Moreover, LGALS3 can undergo post-translational modifications, such as phosphorylation and glycosylation, which can affect its localization, stability, and function.

What is the involvement of LGALS3 in cancer progression and metastasis? 05/13/2018

 LGALS3 has been implicated in various aspects of cancer progression and metastasis. It can promote tumor cell survival, migration, invasion, and angiogenesis. LGALS3 is involved in the modulation of epithelial-mesenchymal transition (EMT), a process essential for cancer cell metastasis. It also interacts with components of the extracellular matrix, promoting tumor cell adhesion and invasion. Furthermore, LGALS3 can influence immune evasion mechanisms employed by cancer cells, contributing to tumor immune escape and resistance to therapy.

Customer Reviews (3)

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Reviews
09/30/2022

    My experimentation flourishes, as if guided by the hands of magic, all thanks to this extraordinary reagent.

    03/18/2020

      Compared to similar products, this reagent demonstrates superior cost-effectiveness, satisfying both in terms of quality and price.

      08/01/2017

        The storage conditions for the reagent are simple and do not require special storage environments.

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