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Recombinant Human HGF, 495-728aa, His-tagged

Cat.No. : HGF-226H
Product Overview : Recombinant Human HGF encoding the human HGF beta chain (Val495–Ser728) was recombined with his tag at the N-Terminus. That was expressed inE.Coli,and purified by Ni-NTA column. MW=26KD.
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  • Gene Information
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Cat. No. : HGF-226H
Description : Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.
Source : E. Coli.
Sequence : Human HGF beta chain (Val495–Ser728)495 VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS 728.
Purity :
>95% in SDS gel.
Formulation : Lyophilized 100 μg human HGF beta in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
Application : Cell biology, ELISA.
Reconstitution : Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
Storage : Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
Gene Name : HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]
Synonyms : HGF; hepatocyte growth factor (hepapoietin A; scatter factor); SF; HGFB; HPTA; F-TCF; DFNB39; scatter factor; hepatopoietin A; OTTHUMP00000206710; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; DFNB39; deafness, autosomal recessive 39; fibroblast-derived tumor cytotoxic factor; scatter factor; Hepatocyte growth factor; SF; Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chainen
Gene ID : 3082
mRNA Refseq : NM_000601
Protein Refseq : NP_000592
MIM : 142409
UniProt ID : P14210
Chromosome Location : 7q21.1
Pathway : Cytokine-cytokine receptor interaction; Focal adhesion; Melanoma; Pathways in cancer; Renal cell carcinoma; Hemostasis
Function : catalytic activity; growth factor activity; protein binding; protein heterodimerization activity; serine-type endopeptidase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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