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Cat. No. |
GTF2H1-1187H
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Description |
TFIIH, a
multisubunit complex is involved in several biological
fundamental mechanisms of the cell: transcription, nucleotide
excision repair, and cell cycle regulation. p62 is one of the
six subunits that constitutes the core of TFIIH. Analysis of the
expression of the p62 gene reveals an over-expression in testis
tissue. This subunit of TFIIH participates in a variety of
protein-protein interactions. For example, Rb competes with TBP
and p62 for binding to E2F thus repressing E2F-mediated
transactivation, herpes simplex virus VP16 and human p53,
directly interact with the p62 subunit of TFIIH. In addition,
TFIIH, via p62 phosphorylation is the major target for mitotic
inactivation of transcription. Recombinant p62 protein is
isolated from an E. coli strain that carries the coding sequence
of human p62 under the control of a T7 promoter. p62 has been
used for protein-protein interactions assays.Protein is greater
than 95% homogeneous and contains no detectable protease, DNase,
and RNase activity. |
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Source |
E. coli. |
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Purity |
> 95% by SDS-PAGE. |
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Form |
Liquid.
Supplied in 20 mM Tris-HCl pH 8.0, 100 Mm KCl, 0.2 mM EDTA, 1 mM
DTT, 20% glycerol. |
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Activity |
100 ng are
sufficient for a protein-protein interaction assay. |
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Storage |
Quality
guaranteed for 12 months. Store at
-80oC. Avoid
freeze / thaw cycles. |
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Usage |
For in vitro
use only. |
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Gene
Information
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Gene Name |
GTF2H1 general transcription factor IIH, polypeptide 1, 62kDa [
Homo sapiens ] |
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Synonyms
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general
transcription factor IIH, polypeptide 1, 62kDa; BTF2; TFB1;
TFIIH; GTF2H1; general transcription factor IIH, polypeptide 1
(62kD subunit); BTF2-p62; Basic transcription factor 2 62 kDa
subunit; General transcription factor IIH polypeptide 1; TFIIH
basal transcription factor complex p62 subunit |
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GeneID |
2965 |
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mRNA Refseq |
NM_001142307 |
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Protein Refseq |
NP_001135779 |
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MIM |
189972 |
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UniProt ID |
P32780 |
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Chromosome
Location |
11p15.1-p14 |
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Pathway |
Basal
transcription factors; Nucleotide excision repair; DNA Repair;
Gene Expression; HIV Infection; Transcription; mRNA Processing |
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Function |
RNA polymerase
II carboxy-terminal domain kinase activity; general RNA
polymerase II transcription factor activity; DNA-dependent
ATPase activity; protein binding; protein kinase activity |
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PDB rendering based on 1pfj.
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