Active Recombinant Human RAD51

• Cat.No.: RAD51-1121H

Specification

• Cat. No.: RAD51-1121H
• Description: Properly controlled recombination between homologous DNA molecules (Homologous Recombination -HR) is essential for the maintenance of genome stability and for the prevention of tumorigenesis. Rad51 is a mammalian homologue of yeast RAD51 and bacterial RecA and, like its counterparts, plays a central role in HR. Rad51 coats ssDNA to form a nucleoprotein filament that invades and pairs with a homologous region in duplex DNA. It can then activate strand exchange to generate a crossover between the juxtaposed DNA molecules. In addition to Rad51, these steps require the coordinated action of a number of other homologous-recombination proteins, including the RP-A protein, which binds single-stranded DNA, Rad52, which can bind DNA ends, anneal complementary single-stranded DNA molecules and enhance the specificity of RAD51, and a number of Rad51 paralogs. The tumour-suppressor proteins BRCA1 and BRCA2 colocalize with RAD51 in DNA-damage-induced nulear foci. BRCA2 has been shown to interact directly with RAD51 and thus plays a direct role in repair by HR, through control of the availability and function of the central mediator, Rad51. Recombinant RAD51 was expressed in a strain of E.coli and purified by an affinity column in combination with FPLC chromatography.
• Source: E.coli.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
• Purity: ≥95% by SDS-PAGE.
• Application: RAD51 has been applied in DNA and protein-protein interaction assays.
• Activity: 20 ng are sufficient for a DNA-protein assay and 100 ng are sufficient for a protein-protein interaction assay.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: RAD51 RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) [ Homo sapiens ]
• Synonyms: RAD51; RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RECA; BRCC5; HRAD51; RAD51A; HsRad51; HsT16930; RAD51 homolog protein; RecA-like protein; RAD51D; recombination protein A; RecA, E. coli, homolog of DNA repair protein RAD51 homolog 1; BRCA1/BRCA2-containing complex, subunit 5; RAD51L3; hRAD51; RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
• Gene ID: 5888
• mRNA Refseq: NM_002875
• Protein Refseq: NP_002866
• MIM: 179617
• UniProt ID: Q06609
• Chromosome Location: 15q15
• Pathway: Homologous recombination; Pancreatic cancer; Pathways in cancer
• Function: ATP binding; damaged DNA binding; double-stranded DNA binding; identical protein binding; nucleoside-triphosphatase activity; nucleotide binding; protein C-terminus binding; single-stranded DNA binding; single-stranded DNA-dependent ATPase activity