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Active Recombinant Human Topoisomerase (DNA) I

Cat.No. : TOP1-1194H
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Cat. No. : TOP1-1194H
Description : Topoisomerase I and IIare the main subtypes of topoisomerase. Human DNA Topoisomerase I is the best studied of the DNA topoisomerase family. Topoisomerase I function includes catalyzing the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA Topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle, and other gene regulatory pathways during cell growth and development. In recent years, topoisomerases have become popular targets for cancer chemotherapy treatments. It is thought that topoisomerase inhibitors block the ligationstep of the cell cycle, generating single and double stranded breaks that harm the integrity of the genome, such as the topotecan used to treat CMML, is a kind oftopoisomerase I inhibitor. Tyrosine 723 was identified as an active site for the DNA binding activity of DNA Topoisomerase I. The covalent intermediate of topo I and DNA complex includes nucleophilic attack by the O4-oxygen of tyrosine 723 on a phosphoester linkage in the DNA. Mutation from tyrosine to phenylalanine at position 723 preferentially binds the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNAs. But mutation at Tyr723 neither affects its kinase activity that phosphorylates splicing factors of SR protein family nor its transcription activity of class II genes in vitro. The mutant Y723F of DNA Topoisomerase I protein (Y723F) was expressed in baculovirus system and purified by using an affinity column and FPLC chromatography. Purified Topo I (Y723F) has been tested for in vitro DNA relaxation assay. Purified mutant Topo I protein (Y723F) is greater than 95% homogeneous and contains no detectable protease, DNase, and RNase activity. Anti-topoisomerase antibodies (ATA) are autoantibodies directed against topoisomerase. Diseases with ATA are autoimmune disease because they react with self-proteins. They are also referred to as anti-DNA topoisomerase I antibody (anti-topo I)
Source : Sf9 insect cells.
Form : Liquid. Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20 % glycerol.
Purity : > 95% by SDS-PAGE.
Activity : 0.1-1 ng of mutated Topo I (Y723F) has been tested for relaxation activity.
Usage : For in vitro use only.
Storage : Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.
Publication :
Suppression of lupus nephritis and skin lesions in MRL/lpr mice by administration of the topoisomerase I inhibitor irinotecan (2016)
Low-Dose Irinotecan Improves Advanced Lupus Nephritis in Mice Potentially by Changing DNA Relaxation and Anti-Double-Stranded DNA Binding (2014)
Gene Name : TOP1 topoisomerase (DNA) I [ Homo sapiens ]
Synonyms : TOP1; topoisomerase (DNA) I; TOPI; DNA topoisomerase1; EC 5.99.1.2; OTTHUMP00000031713; DNA topoisomerase I; topoisomerase I; type I DNA topoisomerase
Gene ID : 7150
mRNA Refseq : NM_003286
Protein Refseq : NP_003277
MIM : 126420
UniProt ID : P11387
Chromosome Location : 20q12-q13.1
Function : ATP binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase type I activity; chromatin binding; nucleotide binding; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the role of TOP1 in the repair of DNA damage caused by environmental factors and chemotherapeutic agents? 12/01/2021

TOP1 repairs DNA damage, maintaining genomic stability under stress.

How do post-translational modifications of TOP1, such as phosphorylation, affect its enzymatic activity and interaction with DNA? 07/17/2021

Post-translational modifications like phosphorylation affect TOP1's activity and DNA interaction.

How do mutations in the TOP1 gene contribute to genomic instability and the development of certain cancers? 02/28/2021

Mutations in TOP1 cause genomic instability, increasing cancer risk.

What are the effects of TOP1 inhibitors on cancer cell proliferation and survival? 12/12/2019

TOP1 inhibitors lead to cancer cell death and inhibit tumor growth by disrupting DNA processes.

How does TOP1 interact with other proteins in the DNA repair and replication machinery? 09/22/2019

TOP1 works with other DNA repair proteins, ensuring accurate DNA synthesis.

How does TOP1 regulate DNA unwinding and re-ligation during transcription and replication? 09/20/2019

TOP1 regulates DNA unwinding by creating transient breaks, aiding transcription and replication.

What are the implications of TOP1 expression levels in predicting the response to chemotherapy in cancer patients? 09/17/2018

TOP1 expression levels predict chemotherapy responses in cancer treatment.

Customer Reviews (3)

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10/17/2019

    Accurate protein structural analysis; a key for structural biology.

    10/06/2018

      High-quality protein crystallography; essential for structural biology.

      05/30/2018

        Timely protein characterization; vital for our research projects.

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